Turn on machines / heaters. Put mice in tailcuff room and let the room and mice acclimate to appropriate temperature for ~30-60 mins. Then, check cuffs for leaks. Put mice into restraints and perform 5 acclimation cycles + 20 recorded cycles.
When placing mice into restraints,
1. Quickly place nose cuff on to avoid letting them turn around in the restraint
2. Make sure that you can see them breathing…
3. Maximize tightness of fit and breathing
Copy the data into a master excel file with two sheets, making sure that there’s only one header (at the very top of the page).
| Specimen Name | Systolic | Mean | Rate | Cycle # | Date | Phase |
|---|---|---|---|---|---|---|
| M1 | 90 | 71 | 711 | 6 | 2021-03-09 | baseline |
| M1 | 110 | 80 | 603 | 7 | 2021-03-09 | baseline |
| M1 | 130 | 86 | 629 | 9 | 2021-03-09 | baseline |
| M1 | 94 | 72 | 693 | 10 | 2021-03-09 | baseline |
| M1 | 97 | 73 | 624 | 12 | 2021-03-09 | baseline |
| M1 | 94 | 72 | 637 | 13 | 2021-03-09 | baseline |
| Specimen Name | Notch | Old Cage ID | New Cage ID | DOB | Body weight (g) | Date | Status | Date of death | Machine ID |
|---|---|---|---|---|---|---|---|---|---|
| M1 | RN | 643254 | 655606 | 2020-12-14 | 27.1 | 2021-03-05 | Alive | NA | 1 |
| M1 | RN | 643254 | 655606 | 2020-12-14 | 26.2 | 2021-03-09 | Alive | NA | 1 |
| M1 | RN | 643254 | 655606 | 2020-12-14 | 25.6 | 2021-03-11 | Alive | NA | 1 |
| M1 | RN | 643254 | 655606 | 2020-12-14 | 24.9 | 2021-03-14 | Alive | NA | 1 |
| M1 | RN | 643254 | 655606 | 2020-12-14 | 25.2 | 2021-03-15 | Alive | NA | 1 |
| M1 | RN | 643254 | 655606 | 2020-12-14 | 24.8 | 2021-03-19 | Alive | NA | 1 |
| M2 | XX | 643254 | 655606 | 2020-12-14 | 0.0 | 2021-03-05 | Dead | 2021-03-05 | 1 |
| M2 | XX | 643254 | 655606 | 2020-12-14 | 0.0 | 2021-03-09 | Dead | 2021-03-05 | 1 |
This workbook is set up to analyze two groups of mice! Just run and enjoy (you’ll probably need to change out drug names…)
| group | Specimen Name | Notch | Old Cage ID | New Cage ID | DOB | Status | Date of death | Machine ID | Average body weight (g) |
|---|---|---|---|---|---|---|---|---|---|
| sunitinib | M1 | RN | 643254 | 655606 | 2020-12-14 | Alive | NA | 1 | 25.63333 |
| vehicle | M3 | NN | 643254 | 655606 | 2020-12-14 | Alive | NA | 1 | 24.10000 |
| vehicle | M4 | DR | 643240 | 655607 | 2020-12-15 | Alive | NA | 1 | 27.95000 |
| sunitinib | M5 | LN | 643240 | 655607 | 2020-12-15 | Alive | NA | 1 | 26.46667 |
| sunitinib | M6 | BN | 643240 | 655607 | 2020-12-15 | Alive | NA | 1 | 26.43333 |
| vehicle | M7 | NN | 643253 | 655605 | 2020-12-18 | Alive | NA | 2 | 24.55000 |
| sunitinib | M9 | DR | 643243 | 655604 | 2020-12-17 | Alive | NA | 2 | 26.08333 |
| sunitinib | M10 | RN | 643243 | 655604 | 2020-12-17 | Alive | NA | 2 | 24.73333 |
### Average animal body weight
| Specimen Name | Date | group | # cycles | reason |
|---|---|---|---|---|
| M1 | 2021-03-12 | sunitinib | 19 | Bladder explosion |
| M10 | 2021-03-13 | sunitinib | 2 | Low cycle count |
| M7 | 2021-03-19 | vehicle | 4 | Low cycle count |
| M9 | 2021-03-19 | sunitinib | 1 | Low cycle count |
Detect outliers using boxplot methods. Boxplots are a popular and an easy method for identifying outliers. There are two categories of outlier: (1) outliers and (2) extreme points. Values above Q3 + 1.5xIQR or below Q1 - 1.5xIQR are considered as outliers. Values above Q3 + 3xIQR or below Q1 - 3xIQR are considered as extreme points (or extreme outliers). Q1 and Q3 are the first and third quartile, respectively. IQR is the interquartile range (IQR = Q3 - Q1).
Alternative method: If the absolute difference (i.e. abnormally high or low) between a measurement and the mean of all the cycles for that day is greater than twice the standard deviation for that day, that measurement is removed. It’s assumed that this measurement is due to animal movement or some other non-biological cause.
Since blood pressure is always measured before oral gavage, baseline goes from 03/08 to 03/11, but vehicle treatment starts on 03/11. Observed effect of treatment is then on 03/12!!
Differences are calculated as change per specimen over each phase
For completeness, here is the time series data of each mouse across each Phase of the experiment:
Since BP is recorded before gavage to reduce stress
These are the average blood pressures of each mice across each Phase of the experiment